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KMID : 1177720180120010009
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Journal of Alternatives to Animal Experiments
2018 Volume.12 No. 1 p.9 ~ p.15
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Establishment Study of SkinEthicTM HCE eye irritation test in Korea
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Park Kyo-Hyun
Kim Bae-Hwan
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Abstract
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The objective of this study is to investigate the utility of reconstructed human corneal epithelium model(SkinEthicTM, HCE) as an alternative method to eye irritation test. We confirmed the pre-check test method for eye irritation test through condition setting of production for freeze-killed epidermis, possible direct MTT reduction with test substances, and colorant properties in test chemicals. And, we conducted eye irritation test using 8 chemicals listed in the proficiency test of the OECD test guideline 492. Summarizing the results above of assessment, it was confirmed that the SkinEthicTM HCE proves that it is so efficient and trustworthy enough to replace the eye irritation test. Taken together, the results indicated that the SkinEthicTM HCE proposed by OECD test guideline 492 is expected to become a well-established method in Korea.
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KEYWORD
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Eye irritation, SkinEthicTM, HCE, OECD test guideline 492
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